Multiplex Real-Time PCR Method for Simultaneous Detection and Differentiation of Goat pox Virus, Sheeppox Virus, and Lumpy Skin Disease Virus

Abstract Background The diseases caused by the Capripoxvirus species have very similar symptoms and are difficult to distinguish clinically. According a recent report, not strictly host specific. Objective This study aimed identify viruses from ovine (include sheep goat) or bovine, which will assist in selecting appropriate vaccine correct measures control diseases. Method Universal primers for all specific probes lumpy skin disease virus, sheeppox goatpox virus were designed analyzed (including goats) bovine species. parameters of system, such as annealing temperatures quantities used, optimized. sensitivity, specificity, reproducibility tested. Results Each probe showed fluorescent signal, with no cross reaction other pathogens that cause those poxviruses. LOD was 102 copies target genome DNA. 557 local clinical samples Ethiopia successfully detected results consistent restriction fragment length polymorphism PCR analysis P32 RPO30 genes gene sequencing. Conclusions optimized real-time detection system has good diagnostic sensitivity specificity can be used rapid effective differential diagnosis these goats, sheep, cattle. Highlights It is method bovine.